Offerings / Stem cell storage bank / Peripheral blood bank
Flow Cytometry Laboratory

About Us

In early **2008 (1387 Iranian calendar)**, the peripheral blood freezing and storage program officially began at Royan Institute to provide services to patients suffering from diseases such as multiple myeloma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, aplastic anemia, acute lymphoblastic leukemia, sickle cell anemia, and more. Since **2017 (1396 Iranian calendar)**, these services have been widely offered nationwide by Royan Stem Cell Technology Company.

What is Peripheral Blood?

Peripheral blood contains various types of blood cells, including red blood cells, white blood cells, platelets, and hematopoietic progenitor cells. Stem cells mobilized into peripheral blood are a useful source for regenerating the hematopoietic and immune systems of a patient after intensive chemotherapy.

Flow Cytometry Laboratory

History

In **1979**, for the first time, peripheral blood stem cell transplantation (PBSCTs) was reported for patients with acute granulocytic leukemia (CGL). These cells were collected in the early stages of the disease and preserved by freezing. When the disease reached the acute phase, high-dose chemotherapy was administered, accompanied by the injection of the patient's own (autologous) peripheral stem cells. The results showed that the stem cells present in human blood were capable of regenerating the hematopoietic system and allowed this system to regain its function. In the **1990s**, the presence and identification of stem cells in human peripheral blood led to hypotheses for the treatment of certain cancers, including that hematopoietic stem cells could exist in the bloodstream and are capable of producing differentiated cells to restore the lost function of aplastic bone marrow.

Services Available at Royan Peripheral Blood Stem Cell Bank

Isolation of RBC, Osteoclast, Osteoblast, Adipose from Bone Marrow
Freezing and Storage of Bone Marrow Stem Cells
Isolation of RBC from Peripheral Blood
Freezing and Storage of Peripheral Blood Stem Cells
Applications

Applications

Autologous peripheral blood stem cell transplantation is widely used in the treatment of cancers that are managed with high-dose chemotherapy. This method is not only used for various blood cancers but also for solid tumors sensitive to chemotherapy, including breast cancer and small cell lung cancer. It has also been shown that sequential injections of peripheral blood stem cells can increase the intensity and efficacy of chemotherapy. Furthermore, autologous transplantation of these cells is used to treat certain diseases, such as **multiple myeloma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, acute myeloid leukemia, neuroblastoma, germ cell tumors**, and various **autoimmune diseases**.

Multiple myeloma accounts for approximately 1% of all cancers and about 10% of all blood cancers. Based on the results of clinical trials by various groups since the mid-1990s, high-dose chemotherapy combined with autologous hematopoietic stem cell transplantation has become a standard treatment for multiple myeloma patients who are eligible for transplantation. Previously, patients with multiple myeloma only received chemotherapy, which only alleviated the disease severity in 10% of patients, and only 5% of patients survived for more than ten years. Studies have shown that autologous peripheral blood hematopoietic stem cell transplantation increases patient survival rates by up to 35%. It seems that in the current century, we are facing an increasing prevalence of various diseases, including different types of cancers. Therefore, there is a need for new and extensive investments in novel treatment methods using new stem cell sources for the treatment of many diseases requiring transplantation. Today, throughout the world, including in American countries through medical centers or private companies (Avera McKennan Hospital & University Health Center and Scripps), European countries such as the UK (NHS Center), North Africa (Netcell), and also in India (Cryo Stemcell), efforts have been made to collect and store peripheral blood stem cells for use in cancer patients undergoing high-dose chemotherapy. At Avera McKennan Hospital & University Health Center, over 200 peripheral blood samples are collected annually from patients (it's worth noting that this method is painless compared to bone marrow aspiration), and their hematopoietic stem cells are separated and cryopreserved for future patient use.

Collaborating Centers

Currently, **Ayatollah Taleghani Hospital Transplant Center**, **Mahak Charity Institute**, **Vali-e-Asr Hospital**, **Children's Medical Center**, **Mofid Hospital**, **Afzalipour Hospital Kerman**, and **Hazrat Ali Asghar Hospital** utilize this treatment method. However, Royan Peripheral Blood Stem Cell Bank will be able to provide services to all transplant centers nationwide that have the necessary readiness.

In which diseases is peripheral blood stem cell preservation recommended?

Disease Name: Hodgkin Lymphoma

Hodgkin lymphoma is a cancer that starts in the cells of the immune system, which is responsible for fighting infections and other diseases. Hodgkin lymphoma develops when a lymphocyte (usually a type of B cell), an immune cell of the body, becomes abnormal. This abnormal cell is called a Reed-Sternberg cell. A Reed-Sternberg cell is a large cell with two or more nuclei, each having a very large nucleolus. To multiply, this cell begins to divide. The new cells, in turn, divide repeatedly, creating more abnormal cells. These abnormal cells don't die at the appropriate time, and they don't protect the body against infections or other diseases. The gradual increase in these extra cells leads to the formation of a tissue mass, known as a gland or tumor, in the lymphatic system.

Disease Name: Hodgkin Lymphoma

Hodgkin lymphoma is a cancer that starts in the cells of the immune system, which is responsible for fighting infections and other diseases. Hodgkin lymphoma develops when a lymphocyte (usually a type of B cell), an immune cell of the body, becomes abnormal. This abnormal cell is called a Reed-Sternberg cell. A Reed-Sternberg cell is a large cell with two or more nuclei, each having a very large nucleolus. To multiply, this cell begins to divide. The new cells, in turn, divide repeatedly, creating more abnormal cells. These abnormal cells don't die at the appropriate time, and they don't protect the body against infections or other diseases. The gradual increase in these extra cells leads to the formation of a tissue mass, known as a gland or tumor, in the lymphatic system.

Disease Name: Multiple Myeloma
Multiple myeloma is a malignant tumor related to B cells, characterized by plasma cell proliferation (above 10%) in the bone marrow. This malignancy is part of plasma cell dyscrasias and is clinically and laboratorily identified by: 1. Anemia; 2. Presence of monoclonal proteins in serum (presence of M-band in serum protein electrophoresis of these individuals, resulting from increased monoclonal gamma globulins), urine (presence of Bence-Jones protein in urine), or both; 3. Bone lesions and/or bone pain; 4. Hypercalcemia; and 5. Renal insufficiency.
Disease Name: Thalassemia
Thalassemia is a genetic disorder in which hemoglobin loses its normal structure, leading to the ineffective production of hemoglobin in the body. As a result, the defective hemoglobin cannot effectively deliver oxygen to the body's organs. Hemoglobin is the oxygen-carrying component in red blood cells. Hemoglobin consists of two parts: heme and two different protein chains called alpha and beta. In thalassemia, the globin chains have a normal structure but are produced in reduced amounts. Consequently, blood cells do not form completely and lack the ability to carry sufficient oxygen, resulting in a type of anemia that begins in infancy and lasts throughout life. Although thalassemia is not a single disorder, it encompasses a group of disorders that affect the human body through similar mechanisms. Understanding the differences between various types of thalassemia is important.
Disease Name: Neuroblastoma
Neuroblastoma is a rare disease in which a solid tumor is formed by specific nerve cells called neuroblasts. Normally, these immature cells grow and mature into normal nerve cells. However, in neuroblastoma, these cells turn into cancerous cells. Neuroblastoma usually starts in the tissue of the adrenal glands, which are triangular-shaped glands located above the kidneys. These glands secrete hormones responsible for controlling heart rate, blood pressure, and other important functions. Like other cancers, neuroblastoma can spread to other parts of the body, such as lymph nodes, skin, liver, and bones.
Disease Name: Aplastic Anemia
This anemia is a rare acquired disorder in which the bone marrow does not produce enough new blood cells. Blood cells are derived from stem cells in the bone marrow. In aplastic anemia, the production of blood cells by stem cells decreases, leading to a deficiency of red blood cells (anemia), white blood cells (leading to an increased risk of infection), and platelets (needed to prevent bleeding and bruising). This anemia is not a type of cancer, but rather it is caused by a reduction in stem cells in the bone marrow and the replacement of bone marrow with fat.
Disease Name: Germ Cell Tumor
A **germ cell tumor** is a growth that originates from reproductive cells. These tumors can be cancerous or non-cancerous. Most germ cell tumors are cancerous, such as **testicular cancer** in men or **ovarian cancer** in women. Some germ cell tumors can also occur in other areas of the body, including the abdomen, brain, and chest, although the reason for this is unknown. Germ cell tumors that occur in areas other than the testes and ovaries (extragonadal germ cell tumors) are very rare. Germ cell tumors generally respond well to available treatments, and many are curable even if diagnosed in later stages.
Disease Name: Fanconi Anemia
**Fanconi anemia** is an autosomal recessive disorder, often affecting more than one family member. It is a type of **hereditary aplastic anemia** characterized by pancytopenia, or a reduction in all blood cell lines including red blood cells, platelets, and white blood cells. This condition typically becomes evident after infancy, especially around 8 years of age. Other developmental anomalies such as hyperpigmentation, short stature, hypogonadism, malformations of the extremities (thumb and radius bones), microcephaly, and malformations of other body organs like the heart and kidneys may also be present.
Disease Name: Ewing's Sarcoma
Ewing's sarcoma is a cancerous tumor that originates from connective tissue. Therefore, wherever connective tissue exists in the body, there's a possibility of Ewing's sarcoma. This is why tissues like bone, muscle, or even fat, which contain connective tissue, can be affected. This tumor can occur in any bone but is most common in the pelvis, thigh, and lower leg. Its prevalence peaks in adolescence and young adulthood.
Disease Name: Acute Lymphoblastic Leukemia
Acute Lymphoblastic Leukemia (ALL) is a hematological malignancy resulting from the proliferation and accumulation of lymphoid progenitors in the bone marrow and other tissues, usually involving B-lymphoblastic cells. This disease is seen in both children and adults, with the highest incidence in children aged 2 to 5 years. The clinical onset of ALL is typically sudden and acute; most affected individuals experience clinical symptoms only a few weeks before seeking medical attention. Common clinical symptoms include fever, fatigue, malaise, joint pain, and increased bleeding.
Disease Name: Acute Myeloid Leukemia
Acute Myeloid Leukemia (AML) is a type of blood cancer and the most common type of acute leukemia in the first months of life. However, it accounts for approximately one-third of acute leukemias during childhood and adolescence, and it is the most common form of acute leukemia in middle-aged and older adults. It originates from blood cells (white blood cells other than lymphocytes). Viruses, chemotherapy radiation, benzene, and smoking are factors that increase its incidence. Its onset is often similar to an acute infection, presenting with signs of granulocytic insufficiency along with ulcers on the mucous membranes in the mouth and throat, and fever. Severe disability and general weakness may occur. Acute Myeloid Leukemia, also known as acute non-lymphocytic leukemia, is a cancer of blood cell lines, an aggressive clonal myeloid neoplasm leading to the accumulation of myeloblasts (immature white blood cell precursor cells) in the bone marrow or blood. The rapid growth of these abnormal cells in the bone marrow interferes with the production of normal blood cells.

Peripheral Blood Bank Brochure

To receive the **cord blood brochure** from Royan Stem Cell Technology Company and get more information, click on the link.

Download Brochure
The following contact methods are available for the Peripheral Blood Stem Cell Bank department:
Phone Number: (+9821) 27635100 (+9821) 27635275
Fax: (+9821) 89781304
Mobile: +989102159211
Email: pbb@rsct.ir
Address: No. 24 (Old 7 & 35), Hafez Sharghi Alley, after Bani Hashem Square, Bani Hashem Street, Resalat Highway, Tehran, Iran.
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